anti-t7 antibodies Search Results


anti-t7 antibody conjugated horseradish peroxidase  (Merck KGaA)
90
Merck KGaA anti-t7 antibody conjugated horseradish peroxidase
Anti T7 Antibody Conjugated Horseradish Peroxidase, supplied by Merck KGaA, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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anti-t7 antibody conjugated horseradish peroxidase - by Bioz Stars, 2026-02
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anti t7 antibody  (Merck & Co)
90
Merck & Co anti t7 antibody
Relationship among MXL‐1, TDPT‐1, ETS‐4, CEBP‐1, and PKA in the regulation of axon injury‐induced svh‐2 expression. Interaction between ETS‐4 and TDPT‐1. COS‐7 cells were transfected with plasmids encoding T7‐TDPT‐1, HA‐ETS‐4 (WT), HA‐ETS‐4(S73E) (SE), and HA‐ETS‐4(S73A) (SA), as indicated. Whole‐cell extracts (WCE) and immunoprecipitated complexes obtained with <t>anti‐T7</t> antibody (IP: T7) were analyzed by immunoblotting (IB). In vitro SUMOylation of ETS‐4. COS‐7 cells were transfected with plasmids encoding HA‐ETS‐4 (WT) and HA‐ETS‐4(K32A; K83A) (2KA), as indicated. Cell lysates were immunoprecipitated with anti‐HA antibody. The immunoprecipitates were subjected to in vitro SUMOylation assays. Reaction mixtures were analyzed by immunoblotting (IB) with anti‐SUMO‐2/3 and anti‐HA antibodies. Arrows mark the SUMOylated‐ETS‐4 bands. Schematic representation of the two putative consensus SUMOylation sites and domain structure in ETS‐4. Domains are shown as follows: a pointed domain (PNT; yellow) and an Ets DNA‐binding domain (ETS; blue). SUMOylation of ETS‐4 in mammalian cells. HEK293 cells were transfected with plasmids encoding HA‐ETS‐4 (WT), HA‐ETS‐4(K32A; K83A) (2KA), T7‐TDPT‐1, His‐SUMO‐1, His‐SUMO‐2, and MXL‐1‐Myc, as indicated. SUMO‐conjugated proteins were isolated by cobalt affinity chromatography. ETS‐4 was detected by immunoblotting with anti‐HA antibody. Whole‐cell extracts (WCE) were analyzed by immunoblotting (IB).
Anti T7 Antibody, supplied by Merck & Co, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/anti t7 antibody/product/Merck & Co
Average 90 stars, based on 1 article reviews
anti t7 antibody - by Bioz Stars, 2026-02
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anti-t7 mab peroxidase conjugate  (Upstate Biotechnology Inc)
90
Upstate Biotechnology Inc anti-t7 mab peroxidase conjugate
Relationship among MXL‐1, TDPT‐1, ETS‐4, CEBP‐1, and PKA in the regulation of axon injury‐induced svh‐2 expression. Interaction between ETS‐4 and TDPT‐1. COS‐7 cells were transfected with plasmids encoding T7‐TDPT‐1, HA‐ETS‐4 (WT), HA‐ETS‐4(S73E) (SE), and HA‐ETS‐4(S73A) (SA), as indicated. Whole‐cell extracts (WCE) and immunoprecipitated complexes obtained with <t>anti‐T7</t> antibody (IP: T7) were analyzed by immunoblotting (IB). In vitro SUMOylation of ETS‐4. COS‐7 cells were transfected with plasmids encoding HA‐ETS‐4 (WT) and HA‐ETS‐4(K32A; K83A) (2KA), as indicated. Cell lysates were immunoprecipitated with anti‐HA antibody. The immunoprecipitates were subjected to in vitro SUMOylation assays. Reaction mixtures were analyzed by immunoblotting (IB) with anti‐SUMO‐2/3 and anti‐HA antibodies. Arrows mark the SUMOylated‐ETS‐4 bands. Schematic representation of the two putative consensus SUMOylation sites and domain structure in ETS‐4. Domains are shown as follows: a pointed domain (PNT; yellow) and an Ets DNA‐binding domain (ETS; blue). SUMOylation of ETS‐4 in mammalian cells. HEK293 cells were transfected with plasmids encoding HA‐ETS‐4 (WT), HA‐ETS‐4(K32A; K83A) (2KA), T7‐TDPT‐1, His‐SUMO‐1, His‐SUMO‐2, and MXL‐1‐Myc, as indicated. SUMO‐conjugated proteins were isolated by cobalt affinity chromatography. ETS‐4 was detected by immunoblotting with anti‐HA antibody. Whole‐cell extracts (WCE) were analyzed by immunoblotting (IB).
Anti T7 Mab Peroxidase Conjugate, supplied by Upstate Biotechnology Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/anti-t7 mab peroxidase conjugate/product/Upstate Biotechnology Inc
Average 90 stars, based on 1 article reviews
anti-t7 mab peroxidase conjugate - by Bioz Stars, 2026-02
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mouse monoclonal anti-t7 antibody  (Amersham Pharmacia Biotech Ltd)
90
Amersham Pharmacia Biotech Ltd mouse monoclonal anti-t7 antibody
Relationship among MXL‐1, TDPT‐1, ETS‐4, CEBP‐1, and PKA in the regulation of axon injury‐induced svh‐2 expression. Interaction between ETS‐4 and TDPT‐1. COS‐7 cells were transfected with plasmids encoding T7‐TDPT‐1, HA‐ETS‐4 (WT), HA‐ETS‐4(S73E) (SE), and HA‐ETS‐4(S73A) (SA), as indicated. Whole‐cell extracts (WCE) and immunoprecipitated complexes obtained with <t>anti‐T7</t> antibody (IP: T7) were analyzed by immunoblotting (IB). In vitro SUMOylation of ETS‐4. COS‐7 cells were transfected with plasmids encoding HA‐ETS‐4 (WT) and HA‐ETS‐4(K32A; K83A) (2KA), as indicated. Cell lysates were immunoprecipitated with anti‐HA antibody. The immunoprecipitates were subjected to in vitro SUMOylation assays. Reaction mixtures were analyzed by immunoblotting (IB) with anti‐SUMO‐2/3 and anti‐HA antibodies. Arrows mark the SUMOylated‐ETS‐4 bands. Schematic representation of the two putative consensus SUMOylation sites and domain structure in ETS‐4. Domains are shown as follows: a pointed domain (PNT; yellow) and an Ets DNA‐binding domain (ETS; blue). SUMOylation of ETS‐4 in mammalian cells. HEK293 cells were transfected with plasmids encoding HA‐ETS‐4 (WT), HA‐ETS‐4(K32A; K83A) (2KA), T7‐TDPT‐1, His‐SUMO‐1, His‐SUMO‐2, and MXL‐1‐Myc, as indicated. SUMO‐conjugated proteins were isolated by cobalt affinity chromatography. ETS‐4 was detected by immunoblotting with anti‐HA antibody. Whole‐cell extracts (WCE) were analyzed by immunoblotting (IB).
Mouse Monoclonal Anti T7 Antibody, supplied by Amersham Pharmacia Biotech Ltd, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/mouse monoclonal anti-t7 antibody/product/Amersham Pharmacia Biotech Ltd
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mouse monoclonal anti-t7 antibody - by Bioz Stars, 2026-02
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anti-t7 antibody  (Affibody)
90
Affibody anti-t7 antibody
Relationship among MXL‐1, TDPT‐1, ETS‐4, CEBP‐1, and PKA in the regulation of axon injury‐induced svh‐2 expression. Interaction between ETS‐4 and TDPT‐1. COS‐7 cells were transfected with plasmids encoding T7‐TDPT‐1, HA‐ETS‐4 (WT), HA‐ETS‐4(S73E) (SE), and HA‐ETS‐4(S73A) (SA), as indicated. Whole‐cell extracts (WCE) and immunoprecipitated complexes obtained with <t>anti‐T7</t> antibody (IP: T7) were analyzed by immunoblotting (IB). In vitro SUMOylation of ETS‐4. COS‐7 cells were transfected with plasmids encoding HA‐ETS‐4 (WT) and HA‐ETS‐4(K32A; K83A) (2KA), as indicated. Cell lysates were immunoprecipitated with anti‐HA antibody. The immunoprecipitates were subjected to in vitro SUMOylation assays. Reaction mixtures were analyzed by immunoblotting (IB) with anti‐SUMO‐2/3 and anti‐HA antibodies. Arrows mark the SUMOylated‐ETS‐4 bands. Schematic representation of the two putative consensus SUMOylation sites and domain structure in ETS‐4. Domains are shown as follows: a pointed domain (PNT; yellow) and an Ets DNA‐binding domain (ETS; blue). SUMOylation of ETS‐4 in mammalian cells. HEK293 cells were transfected with plasmids encoding HA‐ETS‐4 (WT), HA‐ETS‐4(K32A; K83A) (2KA), T7‐TDPT‐1, His‐SUMO‐1, His‐SUMO‐2, and MXL‐1‐Myc, as indicated. SUMO‐conjugated proteins were isolated by cobalt affinity chromatography. ETS‐4 was detected by immunoblotting with anti‐HA antibody. Whole‐cell extracts (WCE) were analyzed by immunoblotting (IB).
Anti T7 Antibody, supplied by Affibody, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/anti-t7 antibody/product/Affibody
Average 90 stars, based on 1 article reviews
anti-t7 antibody - by Bioz Stars, 2026-02
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affinity-purified anti-t7 antibody  (Gramsch Inc)
90
Gramsch Inc affinity-purified anti-t7 antibody
Relationship among MXL‐1, TDPT‐1, ETS‐4, CEBP‐1, and PKA in the regulation of axon injury‐induced svh‐2 expression. Interaction between ETS‐4 and TDPT‐1. COS‐7 cells were transfected with plasmids encoding T7‐TDPT‐1, HA‐ETS‐4 (WT), HA‐ETS‐4(S73E) (SE), and HA‐ETS‐4(S73A) (SA), as indicated. Whole‐cell extracts (WCE) and immunoprecipitated complexes obtained with <t>anti‐T7</t> antibody (IP: T7) were analyzed by immunoblotting (IB). In vitro SUMOylation of ETS‐4. COS‐7 cells were transfected with plasmids encoding HA‐ETS‐4 (WT) and HA‐ETS‐4(K32A; K83A) (2KA), as indicated. Cell lysates were immunoprecipitated with anti‐HA antibody. The immunoprecipitates were subjected to in vitro SUMOylation assays. Reaction mixtures were analyzed by immunoblotting (IB) with anti‐SUMO‐2/3 and anti‐HA antibodies. Arrows mark the SUMOylated‐ETS‐4 bands. Schematic representation of the two putative consensus SUMOylation sites and domain structure in ETS‐4. Domains are shown as follows: a pointed domain (PNT; yellow) and an Ets DNA‐binding domain (ETS; blue). SUMOylation of ETS‐4 in mammalian cells. HEK293 cells were transfected with plasmids encoding HA‐ETS‐4 (WT), HA‐ETS‐4(K32A; K83A) (2KA), T7‐TDPT‐1, His‐SUMO‐1, His‐SUMO‐2, and MXL‐1‐Myc, as indicated. SUMO‐conjugated proteins were isolated by cobalt affinity chromatography. ETS‐4 was detected by immunoblotting with anti‐HA antibody. Whole‐cell extracts (WCE) were analyzed by immunoblotting (IB).
Affinity Purified Anti T7 Antibody, supplied by Gramsch Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/affinity-purified anti-t7 antibody/product/Gramsch Inc
Average 90 stars, based on 1 article reviews
affinity-purified anti-t7 antibody - by Bioz Stars, 2026-02
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anti–t7 tag antibody  (Novartis)
90
Novartis anti–t7 tag antibody
Relationship among MXL‐1, TDPT‐1, ETS‐4, CEBP‐1, and PKA in the regulation of axon injury‐induced svh‐2 expression. Interaction between ETS‐4 and TDPT‐1. COS‐7 cells were transfected with plasmids encoding T7‐TDPT‐1, HA‐ETS‐4 (WT), HA‐ETS‐4(S73E) (SE), and HA‐ETS‐4(S73A) (SA), as indicated. Whole‐cell extracts (WCE) and immunoprecipitated complexes obtained with <t>anti‐T7</t> antibody (IP: T7) were analyzed by immunoblotting (IB). In vitro SUMOylation of ETS‐4. COS‐7 cells were transfected with plasmids encoding HA‐ETS‐4 (WT) and HA‐ETS‐4(K32A; K83A) (2KA), as indicated. Cell lysates were immunoprecipitated with anti‐HA antibody. The immunoprecipitates were subjected to in vitro SUMOylation assays. Reaction mixtures were analyzed by immunoblotting (IB) with anti‐SUMO‐2/3 and anti‐HA antibodies. Arrows mark the SUMOylated‐ETS‐4 bands. Schematic representation of the two putative consensus SUMOylation sites and domain structure in ETS‐4. Domains are shown as follows: a pointed domain (PNT; yellow) and an Ets DNA‐binding domain (ETS; blue). SUMOylation of ETS‐4 in mammalian cells. HEK293 cells were transfected with plasmids encoding HA‐ETS‐4 (WT), HA‐ETS‐4(K32A; K83A) (2KA), T7‐TDPT‐1, His‐SUMO‐1, His‐SUMO‐2, and MXL‐1‐Myc, as indicated. SUMO‐conjugated proteins were isolated by cobalt affinity chromatography. ETS‐4 was detected by immunoblotting with anti‐HA antibody. Whole‐cell extracts (WCE) were analyzed by immunoblotting (IB).
Anti–T7 Tag Antibody, supplied by Novartis, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/anti–t7 tag antibody/product/Novartis
Average 90 stars, based on 1 article reviews
anti–t7 tag antibody - by Bioz Stars, 2026-02
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goat antit7 rna polymerase polyclonal antibody  (American Qualex)
90
American Qualex goat antit7 rna polymerase polyclonal antibody
Relationship among MXL‐1, TDPT‐1, ETS‐4, CEBP‐1, and PKA in the regulation of axon injury‐induced svh‐2 expression. Interaction between ETS‐4 and TDPT‐1. COS‐7 cells were transfected with plasmids encoding T7‐TDPT‐1, HA‐ETS‐4 (WT), HA‐ETS‐4(S73E) (SE), and HA‐ETS‐4(S73A) (SA), as indicated. Whole‐cell extracts (WCE) and immunoprecipitated complexes obtained with <t>anti‐T7</t> antibody (IP: T7) were analyzed by immunoblotting (IB). In vitro SUMOylation of ETS‐4. COS‐7 cells were transfected with plasmids encoding HA‐ETS‐4 (WT) and HA‐ETS‐4(K32A; K83A) (2KA), as indicated. Cell lysates were immunoprecipitated with anti‐HA antibody. The immunoprecipitates were subjected to in vitro SUMOylation assays. Reaction mixtures were analyzed by immunoblotting (IB) with anti‐SUMO‐2/3 and anti‐HA antibodies. Arrows mark the SUMOylated‐ETS‐4 bands. Schematic representation of the two putative consensus SUMOylation sites and domain structure in ETS‐4. Domains are shown as follows: a pointed domain (PNT; yellow) and an Ets DNA‐binding domain (ETS; blue). SUMOylation of ETS‐4 in mammalian cells. HEK293 cells were transfected with plasmids encoding HA‐ETS‐4 (WT), HA‐ETS‐4(K32A; K83A) (2KA), T7‐TDPT‐1, His‐SUMO‐1, His‐SUMO‐2, and MXL‐1‐Myc, as indicated. SUMO‐conjugated proteins were isolated by cobalt affinity chromatography. ETS‐4 was detected by immunoblotting with anti‐HA antibody. Whole‐cell extracts (WCE) were analyzed by immunoblotting (IB).
Goat Antit7 Rna Polymerase Polyclonal Antibody, supplied by American Qualex, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/goat antit7 rna polymerase polyclonal antibody/product/American Qualex
Average 90 stars, based on 1 article reviews
goat antit7 rna polymerase polyclonal antibody - by Bioz Stars, 2026-02
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antibody anti-t7  (Koma Biotech)
90
Koma Biotech antibody anti-t7
Relationship among MXL‐1, TDPT‐1, ETS‐4, CEBP‐1, and PKA in the regulation of axon injury‐induced svh‐2 expression. Interaction between ETS‐4 and TDPT‐1. COS‐7 cells were transfected with plasmids encoding T7‐TDPT‐1, HA‐ETS‐4 (WT), HA‐ETS‐4(S73E) (SE), and HA‐ETS‐4(S73A) (SA), as indicated. Whole‐cell extracts (WCE) and immunoprecipitated complexes obtained with <t>anti‐T7</t> antibody (IP: T7) were analyzed by immunoblotting (IB). In vitro SUMOylation of ETS‐4. COS‐7 cells were transfected with plasmids encoding HA‐ETS‐4 (WT) and HA‐ETS‐4(K32A; K83A) (2KA), as indicated. Cell lysates were immunoprecipitated with anti‐HA antibody. The immunoprecipitates were subjected to in vitro SUMOylation assays. Reaction mixtures were analyzed by immunoblotting (IB) with anti‐SUMO‐2/3 and anti‐HA antibodies. Arrows mark the SUMOylated‐ETS‐4 bands. Schematic representation of the two putative consensus SUMOylation sites and domain structure in ETS‐4. Domains are shown as follows: a pointed domain (PNT; yellow) and an Ets DNA‐binding domain (ETS; blue). SUMOylation of ETS‐4 in mammalian cells. HEK293 cells were transfected with plasmids encoding HA‐ETS‐4 (WT), HA‐ETS‐4(K32A; K83A) (2KA), T7‐TDPT‐1, His‐SUMO‐1, His‐SUMO‐2, and MXL‐1‐Myc, as indicated. SUMO‐conjugated proteins were isolated by cobalt affinity chromatography. ETS‐4 was detected by immunoblotting with anti‐HA antibody. Whole‐cell extracts (WCE) were analyzed by immunoblotting (IB).
Antibody Anti T7, supplied by Koma Biotech, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/antibody anti-t7/product/Koma Biotech
Average 90 stars, based on 1 article reviews
antibody anti-t7 - by Bioz Stars, 2026-02
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rabbit polyclonal anti-t7 polymerase antibody  (Wyeth Lederle Japan)
90
Wyeth Lederle Japan rabbit polyclonal anti-t7 polymerase antibody
Relationship among MXL‐1, TDPT‐1, ETS‐4, CEBP‐1, and PKA in the regulation of axon injury‐induced svh‐2 expression. Interaction between ETS‐4 and TDPT‐1. COS‐7 cells were transfected with plasmids encoding T7‐TDPT‐1, HA‐ETS‐4 (WT), HA‐ETS‐4(S73E) (SE), and HA‐ETS‐4(S73A) (SA), as indicated. Whole‐cell extracts (WCE) and immunoprecipitated complexes obtained with <t>anti‐T7</t> antibody (IP: T7) were analyzed by immunoblotting (IB). In vitro SUMOylation of ETS‐4. COS‐7 cells were transfected with plasmids encoding HA‐ETS‐4 (WT) and HA‐ETS‐4(K32A; K83A) (2KA), as indicated. Cell lysates were immunoprecipitated with anti‐HA antibody. The immunoprecipitates were subjected to in vitro SUMOylation assays. Reaction mixtures were analyzed by immunoblotting (IB) with anti‐SUMO‐2/3 and anti‐HA antibodies. Arrows mark the SUMOylated‐ETS‐4 bands. Schematic representation of the two putative consensus SUMOylation sites and domain structure in ETS‐4. Domains are shown as follows: a pointed domain (PNT; yellow) and an Ets DNA‐binding domain (ETS; blue). SUMOylation of ETS‐4 in mammalian cells. HEK293 cells were transfected with plasmids encoding HA‐ETS‐4 (WT), HA‐ETS‐4(K32A; K83A) (2KA), T7‐TDPT‐1, His‐SUMO‐1, His‐SUMO‐2, and MXL‐1‐Myc, as indicated. SUMO‐conjugated proteins were isolated by cobalt affinity chromatography. ETS‐4 was detected by immunoblotting with anti‐HA antibody. Whole‐cell extracts (WCE) were analyzed by immunoblotting (IB).
Rabbit Polyclonal Anti T7 Polymerase Antibody, supplied by Wyeth Lederle Japan, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/rabbit polyclonal anti-t7 polymerase antibody/product/Wyeth Lederle Japan
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rabbit polyclonal anti-t7 polymerase antibody - by Bioz Stars, 2026-02
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anti-t7 antibody in ib  (Aurion)
90
Aurion anti-t7 antibody in ib
Relationship among MXL‐1, TDPT‐1, ETS‐4, CEBP‐1, and PKA in the regulation of axon injury‐induced svh‐2 expression. Interaction between ETS‐4 and TDPT‐1. COS‐7 cells were transfected with plasmids encoding T7‐TDPT‐1, HA‐ETS‐4 (WT), HA‐ETS‐4(S73E) (SE), and HA‐ETS‐4(S73A) (SA), as indicated. Whole‐cell extracts (WCE) and immunoprecipitated complexes obtained with <t>anti‐T7</t> antibody (IP: T7) were analyzed by immunoblotting (IB). In vitro SUMOylation of ETS‐4. COS‐7 cells were transfected with plasmids encoding HA‐ETS‐4 (WT) and HA‐ETS‐4(K32A; K83A) (2KA), as indicated. Cell lysates were immunoprecipitated with anti‐HA antibody. The immunoprecipitates were subjected to in vitro SUMOylation assays. Reaction mixtures were analyzed by immunoblotting (IB) with anti‐SUMO‐2/3 and anti‐HA antibodies. Arrows mark the SUMOylated‐ETS‐4 bands. Schematic representation of the two putative consensus SUMOylation sites and domain structure in ETS‐4. Domains are shown as follows: a pointed domain (PNT; yellow) and an Ets DNA‐binding domain (ETS; blue). SUMOylation of ETS‐4 in mammalian cells. HEK293 cells were transfected with plasmids encoding HA‐ETS‐4 (WT), HA‐ETS‐4(K32A; K83A) (2KA), T7‐TDPT‐1, His‐SUMO‐1, His‐SUMO‐2, and MXL‐1‐Myc, as indicated. SUMO‐conjugated proteins were isolated by cobalt affinity chromatography. ETS‐4 was detected by immunoblotting with anti‐HA antibody. Whole‐cell extracts (WCE) were analyzed by immunoblotting (IB).
Anti T7 Antibody In Ib, supplied by Aurion, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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anti-t7 antibody in ib - by Bioz Stars, 2026-02
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anti-t7 monoclonal antibody-hrp-conjugate  (Amersham Life Sciences Inc)
90
Amersham Life Sciences Inc anti-t7 monoclonal antibody-hrp-conjugate
Relationship among MXL‐1, TDPT‐1, ETS‐4, CEBP‐1, and PKA in the regulation of axon injury‐induced svh‐2 expression. Interaction between ETS‐4 and TDPT‐1. COS‐7 cells were transfected with plasmids encoding T7‐TDPT‐1, HA‐ETS‐4 (WT), HA‐ETS‐4(S73E) (SE), and HA‐ETS‐4(S73A) (SA), as indicated. Whole‐cell extracts (WCE) and immunoprecipitated complexes obtained with <t>anti‐T7</t> antibody (IP: T7) were analyzed by immunoblotting (IB). In vitro SUMOylation of ETS‐4. COS‐7 cells were transfected with plasmids encoding HA‐ETS‐4 (WT) and HA‐ETS‐4(K32A; K83A) (2KA), as indicated. Cell lysates were immunoprecipitated with anti‐HA antibody. The immunoprecipitates were subjected to in vitro SUMOylation assays. Reaction mixtures were analyzed by immunoblotting (IB) with anti‐SUMO‐2/3 and anti‐HA antibodies. Arrows mark the SUMOylated‐ETS‐4 bands. Schematic representation of the two putative consensus SUMOylation sites and domain structure in ETS‐4. Domains are shown as follows: a pointed domain (PNT; yellow) and an Ets DNA‐binding domain (ETS; blue). SUMOylation of ETS‐4 in mammalian cells. HEK293 cells were transfected with plasmids encoding HA‐ETS‐4 (WT), HA‐ETS‐4(K32A; K83A) (2KA), T7‐TDPT‐1, His‐SUMO‐1, His‐SUMO‐2, and MXL‐1‐Myc, as indicated. SUMO‐conjugated proteins were isolated by cobalt affinity chromatography. ETS‐4 was detected by immunoblotting with anti‐HA antibody. Whole‐cell extracts (WCE) were analyzed by immunoblotting (IB).
Anti T7 Monoclonal Antibody Hrp Conjugate, supplied by Amersham Life Sciences Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/anti-t7 monoclonal antibody-hrp-conjugate/product/Amersham Life Sciences Inc
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anti-t7 monoclonal antibody-hrp-conjugate - by Bioz Stars, 2026-02
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Image Search Results


Relationship among MXL‐1, TDPT‐1, ETS‐4, CEBP‐1, and PKA in the regulation of axon injury‐induced svh‐2 expression. Interaction between ETS‐4 and TDPT‐1. COS‐7 cells were transfected with plasmids encoding T7‐TDPT‐1, HA‐ETS‐4 (WT), HA‐ETS‐4(S73E) (SE), and HA‐ETS‐4(S73A) (SA), as indicated. Whole‐cell extracts (WCE) and immunoprecipitated complexes obtained with anti‐T7 antibody (IP: T7) were analyzed by immunoblotting (IB). In vitro SUMOylation of ETS‐4. COS‐7 cells were transfected with plasmids encoding HA‐ETS‐4 (WT) and HA‐ETS‐4(K32A; K83A) (2KA), as indicated. Cell lysates were immunoprecipitated with anti‐HA antibody. The immunoprecipitates were subjected to in vitro SUMOylation assays. Reaction mixtures were analyzed by immunoblotting (IB) with anti‐SUMO‐2/3 and anti‐HA antibodies. Arrows mark the SUMOylated‐ETS‐4 bands. Schematic representation of the two putative consensus SUMOylation sites and domain structure in ETS‐4. Domains are shown as follows: a pointed domain (PNT; yellow) and an Ets DNA‐binding domain (ETS; blue). SUMOylation of ETS‐4 in mammalian cells. HEK293 cells were transfected with plasmids encoding HA‐ETS‐4 (WT), HA‐ETS‐4(K32A; K83A) (2KA), T7‐TDPT‐1, His‐SUMO‐1, His‐SUMO‐2, and MXL‐1‐Myc, as indicated. SUMO‐conjugated proteins were isolated by cobalt affinity chromatography. ETS‐4 was detected by immunoblotting with anti‐HA antibody. Whole‐cell extracts (WCE) were analyzed by immunoblotting (IB).

Journal: EMBO Reports

Article Title: TDP 2 negatively regulates axon regeneration by inducing SUMO ylation of an Ets transcription factor

doi: 10.15252/embr.201847517

Figure Lengend Snippet: Relationship among MXL‐1, TDPT‐1, ETS‐4, CEBP‐1, and PKA in the regulation of axon injury‐induced svh‐2 expression. Interaction between ETS‐4 and TDPT‐1. COS‐7 cells were transfected with plasmids encoding T7‐TDPT‐1, HA‐ETS‐4 (WT), HA‐ETS‐4(S73E) (SE), and HA‐ETS‐4(S73A) (SA), as indicated. Whole‐cell extracts (WCE) and immunoprecipitated complexes obtained with anti‐T7 antibody (IP: T7) were analyzed by immunoblotting (IB). In vitro SUMOylation of ETS‐4. COS‐7 cells were transfected with plasmids encoding HA‐ETS‐4 (WT) and HA‐ETS‐4(K32A; K83A) (2KA), as indicated. Cell lysates were immunoprecipitated with anti‐HA antibody. The immunoprecipitates were subjected to in vitro SUMOylation assays. Reaction mixtures were analyzed by immunoblotting (IB) with anti‐SUMO‐2/3 and anti‐HA antibodies. Arrows mark the SUMOylated‐ETS‐4 bands. Schematic representation of the two putative consensus SUMOylation sites and domain structure in ETS‐4. Domains are shown as follows: a pointed domain (PNT; yellow) and an Ets DNA‐binding domain (ETS; blue). SUMOylation of ETS‐4 in mammalian cells. HEK293 cells were transfected with plasmids encoding HA‐ETS‐4 (WT), HA‐ETS‐4(K32A; K83A) (2KA), T7‐TDPT‐1, His‐SUMO‐1, His‐SUMO‐2, and MXL‐1‐Myc, as indicated. SUMO‐conjugated proteins were isolated by cobalt affinity chromatography. ETS‐4 was detected by immunoblotting with anti‐HA antibody. Whole‐cell extracts (WCE) were analyzed by immunoblotting (IB).

Article Snippet: The membranes were immunoblotted with anti‐HA antibody (mouse 16B12; BioLegend) or anti‐T7 antibody (mouse T7‐Tag; Merck; or rabbit PM022; MBL), and bound antibodies were visualized with horseradish peroxidase (HRP)‐conjugated antibodies against rabbit or mouse IgG using an HRP chemiluminescent substrate reagent kit (Novex ECL; Invitrogen).

Techniques: Expressing, Transfection, Immunoprecipitation, Western Blot, In Vitro, Binding Assay, Isolation, Affinity Chromatography

COS‐7 cells were transfected with plasmids encoding HA‐ETS‐4 and T7‐TDPT‐1, and cell lysates were immunoprecipitated with anti‐HA antibody. The immunoprecipitates were subjected to in vitro SUMOylation assays. Reaction mixtures were analyzed by immunoblotting (IB) with anti‐SUMO‐2/3, anti‐HA, and anti‐T7 antibodies. Arrows mark the SUMOylated‐ETS‐4 bands.

Journal: EMBO Reports

Article Title: TDP 2 negatively regulates axon regeneration by inducing SUMO ylation of an Ets transcription factor

doi: 10.15252/embr.201847517

Figure Lengend Snippet: COS‐7 cells were transfected with plasmids encoding HA‐ETS‐4 and T7‐TDPT‐1, and cell lysates were immunoprecipitated with anti‐HA antibody. The immunoprecipitates were subjected to in vitro SUMOylation assays. Reaction mixtures were analyzed by immunoblotting (IB) with anti‐SUMO‐2/3, anti‐HA, and anti‐T7 antibodies. Arrows mark the SUMOylated‐ETS‐4 bands.

Article Snippet: The membranes were immunoblotted with anti‐HA antibody (mouse 16B12; BioLegend) or anti‐T7 antibody (mouse T7‐Tag; Merck; or rabbit PM022; MBL), and bound antibodies were visualized with horseradish peroxidase (HRP)‐conjugated antibodies against rabbit or mouse IgG using an HRP chemiluminescent substrate reagent kit (Novex ECL; Invitrogen).

Techniques: Transfection, Immunoprecipitation, In Vitro, Western Blot